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About: Hoffman modulation contrast microscopy     Goto   Sponge   NotDistinct   Permalink

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Hoffman modulation contrast microscopy (HMC microscopy) is an optical microscopy technique for enhancing the contrast in unstained biological specimens. The technique was invented by Robert Hoffman in 1975. Like differential interference contrast microscopy (DIC microscopy), contrast is increased by using components in the light path which convert phase gradients in the specimen into differences in light intensity that are rendered in an image that appears three-dimensional. The 3D appearance may be misleading, as a feature which appears to cast a shadow may not necessarily have a distinct physical geometry corresponding to the shadow. The technique is particularly suitable for optical sectioning at lower magnifications.