Sir Nicholas Mosley (c. 1527 – 12 December 1612), also spelt Mosly and Moseley, was a manufacturer of woolen cloth, who subsequently became lord of the manor of Manchester, and a Lord Mayor of London for the year 1599 to 1600. Nicholas Mosley was born in or near Manchester in c. 1527, supposedly the eldest son of Edward Moseley and his wife Margaret Moseley (née Elcock). With his younger brothers, Oswald (1534-1621), Francis (1535-1570), and Anthonie (1537-1607), he appears to have initiated what became a highly successful business as a woolen manufacturer and merchant.