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About: Total internal reflection fluorescence microscope     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : yago:Whole100003553, within Data Space : dbpedia.demo.openlinksw.com associated with source document(s)

Type: yago:WikicatMicroscopesyago:Artifact100021939yago:Device103183080yago:Instrument103574816yago:Instrumentality103575240yago:Magnifier103709206yago:Microscope103760671yago:Object100002684yago:PhysicalEntity100001930yago:ScientificInstrument104147495yago:Whole100003553 New Facet based on Instances of this Class

A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide. The technique is based on the principle that when excitation light is totally internally reflected in a transparent solid coverglass at its interface with a liquid medium, an electromagnetic field, also known as an evanescent wave, is generated at the solid-liquid interface with the same frequency as the excitation light. The intensity of the evanescent wave exponentially decays with distance from the surface of the solid so that only fluorescent molecules within a few hundr