This article details Burton Albion F.C.'s 2009–10 season in League Two. This season was Burton's inaugural season in the Football League and saw them finish comfortably in mid-table at 13th, challenging for the play-off places throughout the season. However, there was little success in other competitions. Despite reaching the second round of the FA Cup, 5–1 defeats against Reading and Chesterfield condemned the Brewers to defeat in the opening rounds of the League Cup and Football League Trophy.