The 2010 NCAA Division I Women's Lacrosse Championship was the 29th annual single-elimination tournament to determine the national champion of Division I NCAA women's college lacrosse. The first two rounds of the tournament were played at the home fields of higher-seeded teams from May 15–22, and the semifinal and championship rounds were played at Johnny Unitas Stadium in Towson, Maryland from May 28–30. All NCAA Division I women's lacrosse programs were eligible for this championship, and a total of 16 teams were invited to participate.