Anthony Stapley (born 30 August 1590 – buried 31 January 1655) was one of the regicides of King Charles I of England. Stapley was M.P. for New Shoreham (1624–1625), Lewes (1628), Sussex (1640, 1653–1654). He was colonel and governor of Chichester (1642–1645) and signed the death-warrant of Charles I. He was a member of Council of State in 1649–1653, vice-admiral of Sussex in 1650 and a member of interim council and of supreme assembly in 1653.