About: C7 protein

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C7 protein is an engineered zinc finger protein based on the murine ZFP, Zif268 and discovered by Wu et al. in 1994 (published in 1995). It shares the same zinc finger 2 and zinc finger 3 of Zif268, but differs in the sequence of finger 1. It also shares the same DNA target, 5'-GCGTGGGCG-3'. The shared sequences in single letter amino acid codes of fingers 2 and 3 are RSD-H-LTT and RAD-E-RKR (positions -1 through 6 in the alpha helix). Zinc finger 1 has the sequence KSA-D-LKR which provides a 13-fold increase in affinity to the target sequence of the entire ZFP over that of Zif268.

Attributes	Values
rdf:type	yago:Abstraction100002137 yago:Act100030358 yago:Activity100407535 yago:Chemical114806838 yago:Compound114818238 yago:Event100029378 yago:Experiment100639556 yago:Investigation100633864 yago:Macromolecule114944888 yago:Material114580897 yago:Matter100020827 yago:Molecule114682133 yago:OrganicCompound114727670 yago:Part113809207 yago:PhysicalEntity100001930 yago:Protein114728724 yago:PsychologicalFeature100023100 yago:Relation100031921 yago:Research100636921 yago:WikicatGeneticsExperiments yago:Work100575741 yago:YagoPermanentlyLocatedEntity protein yago:ScientificResearch100641820 yago:Substance100019613 yago:Thing100002452 yago:Unit109465459 yago:WikicatEngineeredProteins
rdfs:label	C7 protein (en)
rdfs:comment	C7 protein is an engineered zinc finger protein based on the murine ZFP, Zif268 and discovered by Wu et al. in 1994 (published in 1995). It shares the same zinc finger 2 and zinc finger 3 of Zif268, but differs in the sequence of finger 1. It also shares the same DNA target, 5'-GCGTGGGCG-3'. The shared sequences in single letter amino acid codes of fingers 2 and 3 are RSD-H-LTT and RAD-E-RKR (positions -1 through 6 in the alpha helix). Zinc finger 1 has the sequence KSA-D-LKR which provides a 13-fold increase in affinity to the target sequence of the entire ZFP over that of Zif268. (en)
dct:subject	Molecular genetics Engineered proteins Genetics experiments
Wikipage page ID	3930356 (xsd:integer)
Wikipage revision ID	1040083558 (xsd:integer)
Link from a Wikipage to another Wikipage	Molecular genetics Amino acid Aspartic acid Engineered proteins Zinc finger Genetics experiments C7.GAT protein Protein engineering Zif268 Zinc finger protein
sameAs	C7 protein C7 protein C7 protein C7 protein
dbp:wikiPageUsesTemplate	dbt:Cite_journal dbt:Short_description dbt:Transcription_factors_and_intracellular_receptors
has abstract	C7 protein is an engineered zinc finger protein based on the murine ZFP, Zif268 and discovered by Wu et al. in 1994 (published in 1995). It shares the same zinc finger 2 and zinc finger 3 of Zif268, but differs in the sequence of finger 1. It also shares the same DNA target, 5'-GCGTGGGCG-3'. The shared sequences in single letter amino acid codes of fingers 2 and 3 are RSD-H-LTT and RAD-E-RKR (positions -1 through 6 in the alpha helix). Zinc finger 1 has the sequence KSA-D-LKR which provides a 13-fold increase in affinity to the target sequence of the entire ZFP over that of Zif268. It is used in zinc finger investigations in which the amino acid sequence of finger 2 is changed in order to determine the appropriate sequence to target a given three-nucleotide target site. A variation of C7, C7.GAT is preferred since it lacks the aspartic acid residue present in finger 3 of C7 and known to cause a phenomenon called 'target site overlap'. In this case the target site overlap is a result of the aspartic acid residue forming a hydrogen bond with the N4 of the cytosine (in the opposite strand) base-paired to the guanine in the finger 2 subsite. It can also form the same hydrogen bond with an adenine base paired to a thymine. This target site overlap would dictate that either a cytosine or adenine residue be present as the 3' nucleotide in the finger 2 subsite which is unacceptable when looking to target sequences containing another nucleotide at this position. (en)
gold:hypernym	Protein
prov:wasDerivedFrom	wikipedia-en:C7_protein?oldid=1040083558&ns=0
page length (characters) of wiki page	2108 (xsd:nonNegativeInteger)
foaf:isPrimaryTopicOf	wikipedia-en:C7_protein
is Link from a Wikipage to another Wikipage of	C7 Thrombospondin Zinc finger chimera Sushi domain C7.GAT protein
is foaf:primaryTopic of	wikipedia-en:C7_protein

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