Ebenezer Mattoon (August 19, 1755 – September 11, 1843) was a United States representative from Massachusetts. He was born in North Amherst in the Province of Massachusetts Bay on August 19, 1755. He attended the common schools and received private instruction. He graduated from Dartmouth College in 1776. Mattoon served in the Revolutionary Army. He taught school and also engaged in agricultural pursuits. He was a delegate to the state constitutional convention in 1820. Mattoon died in Amherst on September 11, 1843. His interment was in West Cemetery.