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High Five (BTI-Tn-5B1-4) is an insect cell line that originated from the ovarian cells of the cabbage looper, Trichoplusia ni. It was developed by the Boyce Thompson Institute for Plant Research. High Five cells have become one of the most commonly used cell lines for recombinant protein expression using baculovirus or transfection, and have been demonstrated to express more recombinant protein than other lepidopteran cell lines, such as Sf9 cells. The High Five cells have been used to produce the VLP-based HPV vaccine Cervarix.

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  • High Five cells (en)
  • High Five細胞 (zh)
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  • High Five（正式名稱為BTI-Tn-5B1-4）是一種昆蟲細胞系，起源於的卵巢細胞。 它是由美國的植物研究所發現的。High Five 細胞是使用桿狀病毒或轉染，進行重組蛋白表達的其中一個最常用細胞系，並且比Sf9細胞等鱗翅類昆蟲細胞系表達更多的重組蛋白。它們可以在沒有血清的情況下生長，並且可以以鬆散的附著狀態或懸濁液培養 。High Five細胞可產生大量的小分子核糖核酸、小分子干擾核糖核酸及piRNA，使其適合於研究以上三種類型的小沉默核糖核酸。 (zh)
  • High Five (BTI-Tn-5B1-4) is an insect cell line that originated from the ovarian cells of the cabbage looper, Trichoplusia ni. It was developed by the Boyce Thompson Institute for Plant Research. High Five cells have become one of the most commonly used cell lines for recombinant protein expression using baculovirus or transfection, and have been demonstrated to express more recombinant protein than other lepidopteran cell lines, such as Sf9 cells. The High Five cells have been used to produce the VLP-based HPV vaccine Cervarix. (en)
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  • High Five (BTI-Tn-5B1-4) is an insect cell line that originated from the ovarian cells of the cabbage looper, Trichoplusia ni. It was developed by the Boyce Thompson Institute for Plant Research. High Five cells have become one of the most commonly used cell lines for recombinant protein expression using baculovirus or transfection, and have been demonstrated to express more recombinant protein than other lepidopteran cell lines, such as Sf9 cells. The High Five cells have been used to produce the VLP-based HPV vaccine Cervarix. They can be grown in the absence of serum, and can be cultured in a loose attached state or in suspension High Five cells produce abundant microRNAs (miRNAs), small interfering RNAs (siRNAs), and PIWI-interacting RNAs (piRNAs), making it suitable to study all three types of small silencing RNAs. The High Five cell line, like other cell lines, has been found to host a non-pathogenic adventitious alphanodavirus. Researchers at the Boyce Thompson Institute were able to isolate virus-free sub-clones, named Tnao38 and Tnms42. (en)
  • High Five（正式名稱為BTI-Tn-5B1-4）是一種昆蟲細胞系，起源於的卵巢細胞。 它是由美國的植物研究所發現的。High Five 細胞是使用桿狀病毒或轉染，進行重組蛋白表達的其中一個最常用細胞系，並且比Sf9細胞等鱗翅類昆蟲細胞系表達更多的重組蛋白。它們可以在沒有血清的情況下生長，並且可以以鬆散的附著狀態或懸濁液培養 。High Five細胞可產生大量的小分子核糖核酸、小分子干擾核糖核酸及piRNA，使其適合於研究以上三種類型的小沉默核糖核酸。 (zh)
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