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About: MOWChIP-seq     Goto   Sponge   NotDistinct   Permalink

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MOWChIP-seq (Microfluidic Oscillatory Washing–based Chromatin ImmunoPrecipitation followed by sequencing) is a microfluidic technology used in molecular biology for profiling genome-wide histone modifications and other molecular bindings using as few as 30-100 cells per assay. MOWChIP-seq is a special type of ChIP-seq assay designed for low-input and high-throughput assays. The overall process of MOWChIP-seq is similar to that of conventional ChIP-seq assay except that the chromatin immunoprecipitation (ChIP) and washing steps occur in a small microfluidic chamber. MOWChIP-seq takes advantage of the capability of microfluidics for manipulating micrometer-sized beads. In the process, a packed bed of beads is formed to drastically increase the adsorption efficiency of chromatin fragments. An