Sir John Borlase, 2nd Baronet (1642 – 1 February 1689) was an English politician. Born in Bockmer End in Buckinghamshire, he was the son of Sir John Borlase, 1st Baronet and Alice Bankes, daughter of Sir John Bankes, Lord Chief Justice of the Court of Common Pleas and Mary Hawtrey. His nephew was Borlase Warren. Borlase died unmarried and was buried in Stratton Audley in Oxfordshire. With his death the baronetcy became extinct. His estate passed eventually to his nephew Borlase Warren, who sat as MP for Nottingham in four Parliaments between 1713 and 1747.