About: Total internal reflection fluorescence microscope

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A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide. The technique is based on the principle that when excitation light is totally internally reflected in a transparent solid coverglass at its interface with a liquid medium, an electromagnetic field, also known as an evanescent wave, is generated at the solid-liquid interface with the same frequency as the excitation light. The intensity of the evanescent wave exponentially decays with distance from the surface of the solid so that only fluorescent molecules within a few hundr

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rdfs:label	Interne Totalreflexionsfluoreszenzmikroskopie (de) Microscopio de fluorescencia de reflexión interna total (es) Microscope de fluorescence par réflexion totale interne (fr) 全反射照明蛍光顕微鏡 (ja) Mikroskopia fluorescencyjna całkowitego wewnętrznego odbicia (pl) Total internal reflection fluorescence microscope (en) 全內反射螢光顯微鏡 (zh)
rdfs:comment	Interne Totalreflexionsfluoreszenzmikroskopie (englisch total internal reflection fluorescence microscopy, TIRFM) ist eine spezielle Methode der Lichtmikroskopie, bei der die Fluoreszenz eines Präparats über ein evaneszentes (abklingendes) Feld angeregt wird. Zur Erzeugung des evaneszenten Feldes wird Licht an der Innenseite eines Reflexionselement (z. B. ein Deckglas oder ein Reflexionsprisma) an der Grenzfläche zum Präparat totalreflektiert. Die Beschränkung auf Beobachtungen nahe dem Deckglas hat TIRF-Mikroskopie mit Interferenzreflexionsmikroskopie gemeinsam. (de) Un microscopio de fluorescencia de reflexión interna total (en inglés, Total internal reflection fluorescence microscope o TIRFM) es un tipo de microscopio con el cual una fina región de una muestra, usualmente de menos de 200 nm de espesor, puede ser observada. (es) 全反射照明蛍光顕微鏡（ぜんはんしゃしょうめいけいこうけんびきょう、Total internal reflection fluorescence microscope）、TIRF（Total Internal Reflection Fluorescence）顕微鏡、エバネッセント場顕微鏡とは、カバーガラスなどの全反射面の裏側にトンネル効果によりしみだすエバネッセント光を励起光源とした顕微鏡である。拡散しない光源が必要なため、通常レーザー光源が用いられる（オリンパス社には楔形プリズムを使用した、アーク光源のシステムも存在する）。 (ja) 全内反射荧光显微镜（total internal reflection fluorescent microscope，TIRFM），利用光線全反射後在介質另一面產生的特性，激發荧光分子以觀察螢光標定樣品的極薄區域，觀測的動態範圍通常在200nm以下。因為激發光呈指數衰減的特性，只有極靠近全反射面的樣本區域會產生螢光反射，大大降低了背景光雜訊干擾觀測標的，故此項技術廣泛應用於細胞表面物質的動態觀察。 (zh) Le microscope de fluorescence par réflexion totale interne (TIRFM, total internal reflection fluorescence microscopy), ou microscope à onde évanescente, est un type particulier de microscope optique à fluorescence permettant d'examiner une tranche très fine d'un échantillon (moins de 200 nm d'épaisseur), grâce à un mode d'illumination particulier : la réflexion totale interne. (fr) A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide. The technique is based on the principle that when excitation light is totally internally reflected in a transparent solid coverglass at its interface with a liquid medium, an electromagnetic field, also known as an evanescent wave, is generated at the solid-liquid interface with the same frequency as the excitation light. The intensity of the evanescent wave exponentially decays with distance from the surface of the solid so that only fluorescent molecules within a few hundr (en) Mikroskopia fluorescencyjna całkowitego wewnętrznego odbicia (ang. Total Internal Reflection Fluorescence Microscope (TIRFM) – odmiana mikroskopii fluorescencyjnej pozwalająca na obrazownie próbek tylko do określonej głębokości, zwykle do 200 nanometrów w głąb. W ten sposób w TIRFM następuje bardzo selektywna wizualizacja obszarów położonych zaraz pod szkiełkiem lub pryzmatem (może to być błona nakrytej nim komórki), ponieważ obszar wzbudzenia powstaje tylko w zasięgu fali zanikającej, co znacznie ogranicza wpływ tła (wnętrza komórki). Znacznie powiększa to rozdzielczość uzyskanych obrazów. (pl)
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dct:subject	Fluorescence techniques American inventions Cell imaging Laboratory equipment Microscopes Optical microscopy techniques Scientific techniques
Wikipage page ID	1183118 (xsd:integer)
Wikipage revision ID	1118102534 (xsd:integer)
Link from a Wikipage to another Wikipage	Electromagnetic field Molecule Plasma membrane Deconvolution Neurotransmitter Confocal microscopy Convolution Fluorescence techniques Objective (optics) Observation American inventions Hormone Total internal reflection Cell imaging Laboratory equipment Microscopes Optical microscopy techniques Dynamic equilibrium Evanescent wave Exponential decay Numerical aperture Cell adhesion Cell biology Fluorescence microscope Fluorophore Spatial resolution Covalent bond Scientific techniques Biophysics Stray light Autofluorescence Poisson distribution Microscope Photobleaching Maximum likelihood sequence estimation Snell's law Secretion Evanescent field Molecular biology Single-molecule experiment Visible light Visualization (graphic)
Link from a Wikipage to an external page	http://www.lambertinstruments.com/en/flim/lifa-combined-to-laser-tirf.html http://www.microscopy.olympus.eu/microscopes/Life_Science_Microscopes_TIRFM.htm http://www.tirf-labs.com/tirfmicroscopy.html http://www.microscopyu.com/articles/fluorescence/tirf/tirfintro.html http://www.olympusmicro.com/primer/techniques/fluorescence/tirf/tirfhome.html http://www.zeiss.de/c12567be0045acf1/Contents-Frame/649722ef8c671420c1256dd6004b7751 https://deepblue.lib.umich.edu/bitstream/2027.42/72779/1/j.1600-0854.2001.21104.x.pdf%7Cpmid=11733042%7Chdl=2027.42/72779%7Cs2cid=15202097%7Chdl-access=free https://www.micro-shop.zeiss.com/%3Fs=2525647761b33&l=en&p=us&f=f http://www.colorado.edu/schwartzgroup/
sameAs	Total internal reflection fluorescence microscope Total internal reflection fluorescence microscope Total internal reflection fluorescence microscope Total internal reflection fluorescence microscope Total internal reflection fluorescence microscope Total internal reflection fluorescence microscope Total internal reflection fluorescence microscope Total internal reflection fluorescence microscope Total internal reflection fluorescence microscope Total internal reflection fluorescence microscope
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