The 1927 Preakness Stakes was the 52nd running of the $63,100 Preakness Stakes Thoroughbred horse race. The race took place on May 9, 1927, and was won by Bostonian who was ridden by Whitey Abel. The colt won the race by a half length over runner-up Sir Harry. The mile and three sixteenths race was run on a track rated good in a final time of 2:01 3/5.