The 1965 NCAA University Division basketball tournament involved 23 schools playing in single-elimination play to determine the national champion of men's NCAA Division I college basketball. It began on March 8, 1965, and ended with the championship game on March 20 in Portland, Oregon. A total of 27 games were played, including a third-place game in each region and a national third-place game. Of note, this was the last NCAA Tournament for Henry Iba of Oklahoma State.