The 2001 Exxon Superflo 12 Hours of Sebring was the 49th running of this event. It was both the second round of the 2001 American Le Mans Series season and the opening round of the new European Le Mans Series. It took place at Sebring International Raceway, Florida, on March 17, 2001. This would not only be the final victory of Michele Alboreto's lifetime but also his final race ever as he died one month later.