The 2001 Big 12 Conference Baseball Tournament was held at AT&T Bricktown Ballpark in Oklahoma City, OK from May 17 through May 21. Nebraska won their third tournament in a row and earned the Big 12 Conference's automatic bid to the 2001 NCAA Division I Baseball Tournament. The tournament mirrored the format of the College World Series, with two 4-team double-elimination brackets and a final championship game. It was also the final appearance for Iowa State in the tournament, as they discontinued their baseball program following the season.