The 2016–17 North Florida Ospreys women's basketball team represented the University of North Florida in the 2016–17 NCAA Division I women's basketball season. The Ospreys, led by second year head coach , played their games at UNF Arena and were members of the Atlantic Sun Conference. They finished the season 10–20, 3–11 in A-Sun play to finish in a tie for sixth place. They lost in the quarterfinals of to Florida Gulf Coast.