The 2021 Louisville Cardinals women's soccer team represented University of Louisville during the 2021 NCAA Division I women's soccer season. The Cardinals were led by head coach Karen Ferguson-Dayes, in her twenty-second season. They played home games at Lynn Stadium. This was the team's 37th season playing organized women's college soccer and their 8th playing in the Atlantic Coast Conference. The Cardinals finished the season 7–7–2 overall and 3–6–1 in ACC play to finish in eleventh place. The team did not qualify for the ACC Tournament and were not invited to the NCAA Tournament.