State Route 101 (SR 101) is a 39.107-mile-long (62.937 km) north–south state highway in the northwestern part of the U.S. state of Alabama. The southern terminus of the highway is at an intersection with (CR 460), the former route of SR 24 west of Moulton. The highway continues northward to the Tennessee state line, where it continues as Tennessee State Route 227 (SR 227).