Ascot Vale railway station is located on the Craigieburn line in Victoria, Australia. It serves the northern Melbourne suburb of Ascot Vale, and it opened on 1 November 1860. The station opened as part of the private Melbourne and Essendon Railway Company line to Essendon. The station closed with the line on 1 July 1864, but was reopened on 9 October 1871, under government ownership.