About: Comet assay     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : yago:Trial105799212, within Data Space : dbpedia.demo.openlinksw.com associated with source document(s)
QRcode icon
http://dbpedia.demo.openlinksw.com/describe/?url=http%3A%2F%2Fdbpedia.org%2Fresource%2FComet_assay&invfp=IFP_OFF&sas=SAME_AS_OFF

The single cell gel electrophoresis assay (SCGE, also known as comet assay) is an uncomplicated and sensitive technique for the detection of DNA damage at the level of the individual eukaryotic cell. It was first developed by Östling & Johansson in 1984 and later modified by Singh et al. in 1988. It has since increased in popularity as a standard technique for evaluation of DNA damage/repair, biomonitoring and genotoxicity testing. It involves the encapsulation of cells in a low-melting-point agarose suspension, lysis of the cells in neutral or alkaline (pH>13) conditions, and electrophoresis of the suspended lysed cells. The term "comet" refers to the pattern of DNA migration through the electrophoresis gel, which often resembles a comet.

AttributesValues
rdf:type
rdfs:label
  • اختبار المذنب (ar)
  • Comet-Assay (de)
  • Comet assay (en)
  • Test della cometa (it)
  • Test des comètes (fr)
  • コメットアッセイ (ja)
  • Comet-assay (nl)
  • Ensaio cometa (pt)
  • Метод ДНК-комет (ru)
  • 彗星实验 (zh)
rdfs:comment
  • اختبار المذنب رحلان كهربي هلامي لخلية واحدة (المعروف أيضا باسم المذنب) هي تقنية غير معقدة وحساسة للكشف عن أضرار الحمض النووي على مستوى فردي للخلية الحقيقية النواة. وكان أول من وصف الاختبار هو سينغ وآخرون في عام 1988. ومنذ ذلك الحين اكتسب هذا الاختبار شعبية قياسية كتقنية للحمض النووي لتقييم الاضرار/إصلاح، والرصد الحيوي وتجارب السمية الجينية. وهو يشتمل على تغليف الخلايا في اغاروز منخفض ذو درجة ذوبان منخفضة وثم تحلل الخلايا في ظروف معتدلة أو قاعدية (pH>13)و بعدها إجراء الرحلان الكهربائي.و بعد ذلك يحلل الدنا المصبغ لتحديد الضرر به.هذا ويمكن أن يقوم بهذه الطريقة يدويا أو اوتوماتيكيا بواسطة برنامج على الحاسوب. (ar)
  • Le test des comètes (Comet assay, en anglais) est une technique d'électrophorèse sur gel d'agarose. Il permet de mesurer les cassures induites directement par un agent génotoxique et indirectement lors des processus enzymatiques de réparation des dommages ou lors de processus secondaires de fragmentation de l'ADN tel que l'apoptose. (fr)
  • Il test della cometa, noto anche come elettroforesi su singola cellula, è un test di mutagenesi per l'identificazione di danni al DNA in una cellula, solitamente di mammifero. Il test è conosciuto anche con le dizioni inglesi comet assay, Single Cell Gel Electrophoresis e la sua sigla SCGE. (it)
  • コメットアッセイ(comet assay)は変異原性試験の一種。電気泳動の原理を利用し真核生物の細胞または細胞核におけるDNAの切断を検出する方法で、単細胞ゲル電気泳動法(Single cell gel electrophoresis;SCGE)とも呼ばれる。DNAの損傷から修復の過程を指標として変異原性(遺伝毒性)を調べる方法としてよく用いられる。またアポトーシスの検出にも用いられる。 (ja)
  • O ensaio de eletroforese em gel de célula única (SCGE, também conhecido como ensaio cometa) é uma técnica simples e sensível para a detecção de danos no DNA no nível da célula eucariótica individual. Foi desenvolvido pela primeira vez por Östling & Johansson em 1984 e posteriormente modificado por Singh et al. em 1988. Desde então, aumentou em popularidade como uma técnica padrão para avaliação de danos/reparos de DNA, biomonitoramento e testes de genotoxicidade. Envolve o encapsulamento das células em uma suspensão de agarose de baixo ponto de fusão, a lise das células em condições neutras ou alcalinas (pH > 13) e a eletroforese das células lisadas suspensas. O termo "cometa" refere-se ao padrão de migração de DNA através do gel de eletroforese, que muitas vezes se assemelha a um cometa. (pt)
  • Метод ДНК-комет — это метод регистрации повреждения ДНК и изучения репарации на уровне одиночных клеток. Метод способен выявлять разрывы нитей ДНК в отдельных клетках. Является быстрым и весьма чувствительным. (ru)
  • 彗星实验(英語:comet assay),又称作单细胞凝胶电泳实验或彗星电泳法(英語:Single Cell Gel Electrophoresis assay,SCGE)是一种在单个真核细胞水平上检测DNA损伤的实验技术。彗星实验最初由Östling和Johansson在1984年发明,此后由 Singh等在1988年改进,此后成为了间的DNA损伤与修复、遗传毒性生物监测的最常用的标准实验技术。It involves the encapsulation of cells in a low-melting-point agarose suspension, lysis of the cells in neutral or alkaline (pH>13) conditions, and electrophoresis of the suspended lysed cells. The term "comet" refers to the pattern of DNA migration through the electrophoresis gel, which often resembles a comet. (zh)
  • Der Comet-Assay (auch Einzelzellgelelektrophorese genannt) ist eine Technik der Gelelektrophorese, durch die es ermöglicht wird, DNA-Schädigungen in einzelnen Zellen festzustellen. Entwickelt wurde der Assay 1984 von Östling und Johanson zum Nachweis von DNA-Doppelstrangbrüchen. Mit der Weiterentwicklung durch Singh im Jahre 1988 konnten durch die Verwendung von basischen Puffern zusätzlich auch DNA-Einzelstrangbrüche festgestellt werden. Beim Comet-Assay können alle Zellen verwendet werden, die einen Zellkern besitzen. (de)
  • The single cell gel electrophoresis assay (SCGE, also known as comet assay) is an uncomplicated and sensitive technique for the detection of DNA damage at the level of the individual eukaryotic cell. It was first developed by Östling & Johansson in 1984 and later modified by Singh et al. in 1988. It has since increased in popularity as a standard technique for evaluation of DNA damage/repair, biomonitoring and genotoxicity testing. It involves the encapsulation of cells in a low-melting-point agarose suspension, lysis of the cells in neutral or alkaline (pH>13) conditions, and electrophoresis of the suspended lysed cells. The term "comet" refers to the pattern of DNA migration through the electrophoresis gel, which often resembles a comet. (en)
  • Comet-assay en single-cell gel electrophoresis assay (SCGE) zijn Engelse termen voor een eenvoudige en gevoelige techniek om schade aan het DNA op te sporen in individuele eukaryotische cellen. De techniek werd in 1984 ontwikkeld door Östling en Johansson en in 1988 verbeterd door Singh et al. Sindsdien is deze test een standaardtechniek geworden voor het beoordelen van de schade aan en de reparatie van DNA, die wordt gebruikt bij biomonitoring en het testen van gentoxiciteit. (nl)
foaf:depiction
  • http://commons.wikimedia.org/wiki/Special:FilePath/Casplabapp.jpg
dcterms:subject
Wikipage page ID
Wikipage revision ID
Link from a Wikipage to another Wikipage
Link from a Wikipage to an external page
sameAs
Faceted Search & Find service v1.17_git139 as of Feb 29 2024


Alternative Linked Data Documents: ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 08.03.3330 as of Mar 19 2024, on Linux (x86_64-generic-linux-glibc212), Single-Server Edition (378 GB total memory, 53 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software