About: FLP-FRT recombination

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In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo. It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 µ plasmid of baker's yeast Saccharomyces cerevisiae. The 34bp minimal FRT site sequence has the sequence 5'GAAGTTCCTATTCtctagaaaGtATAGGAACTTC3'

Attributes	Values
rdf:type	company
rdfs:label	Flp/FRT-System (de) FLP-FRT recombination (en)
rdfs:comment	Das Flp/FRT-System ist eine Methode der Genetik zur Entfernung oder Einfügung von DNA-Sequenzen. Das Flp/FRT-System wird unter anderem in der reversen Genetik und im Kassettenaustauschverfahren zur Erzeugung transgener Organismen verwendet. (de) In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo. It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 µ plasmid of baker's yeast Saccharomyces cerevisiae. The 34bp minimal FRT site sequence has the sequence 5'GAAGTTCCTATTCtctagaaaGtATAGGAACTTC3' (en)
name	Site-specific recombinase Flp (en)
foaf:depiction
dct:subject	Molecular genetics Genetics techniques
Wikipage page ID	6019108 (xsd:integer)
Wikipage revision ID	1060774978 (xsd:integer)
Link from a Wikipage to another Wikipage	Mosaic (genetics) Thermolabile Holliday junction DNA replication Saccharomyces cerevisiae Northern blot Molecular genetics Genetic recombination Genetics Frontotemporal dementia Mutagenesis GAL4/UAS system Amyotrophic lateral sclerosis Baker's yeast dbr:Tamoxifen Cre-Lox recombination Cre recombinase Genetics techniques Homologous recombination Recombinase Recombinase-mediated cassette exchange Green fluorescent protein Site-specific recombination Schwann cell Site-specific recombinase technology Reverse transcription polymerase chain reaction
Link from a Wikipage to an external page	https://www.youtube.com/watch%3Fv=JLQ5sNrJ-lk http://www.jove.com/video/3761/transflp-method-to-genetically-modify-vibrio-cholerae-based-on http://www.jove.com/video/50610/the-tomatogfp-flpfrt-method-for-live-imaging-mosaic-adult-drosophila
sameAs	FLP-FRT recombination FLP-FRT recombination FLP-FRT recombination FLP-FRT recombination
dbp:wikiPageUsesTemplate	dbt:Infobox_nonhuman_protein dbt:Reflist dbt:DNA_sequence
thumbnail	wiki-commons:Special:FilePath/Simplified_Flp-FRT_Recombinase_Mechanism.png?width=300
Organism	Saccharomyces cerevisiae (en)
symbol	FLP1 (en)
UniProt	P03870 (en)
has abstract	Das Flp/FRT-System ist eine Methode der Genetik zur Entfernung oder Einfügung von DNA-Sequenzen. Das Flp/FRT-System wird unter anderem in der reversen Genetik und im Kassettenaustauschverfahren zur Erzeugung transgener Organismen verwendet. (de) In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo. It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 µ plasmid of baker's yeast Saccharomyces cerevisiae. The 34bp minimal FRT site sequence has the sequence 5'GAAGTTCCTATTCtctagaaaGtATAGGAACTTC3' for which flippase (Flp) binds to both 13-bp 5'-GAAGTTCCTATTC-3' arms flanking the 8 bp spacer, i.e. the site-specific recombination (region of crossover) in reverse orientation. FRT-mediated cleavage occurs just ahead from the asymmetric 8bp core region (5'tctagaaa3') on the top strand and behind this sequence on the bottom strand. Several variant FRT sites exist, but recombination can usually occur only between two identical FRTs but generally not among non-identical ("heterospecific") FRTs. (en)
gold:hypernym	Technology
prov:wasDerivedFrom	wikipedia-en:FLP-FRT_recombination?oldid=1060774978&ns=0
page length (characters) of wiki page	20701 (xsd:nonNegativeInteger)
foaf:isPrimaryTopicOf	wikipedia-en:FLP-FRT_recombination
is Link from a Wikipage to another Wikipage of	FLP-FRT Recombination Mosaic (genetics) MARCM DNA adenine methyltransferase identification International Mouse Phenotyping Consortium Gene targeting Genetic engineering techniques Genome editing Cell lineage Floxing Isogenic human disease models Flippase recognition target Cre-Lox recombination Cre recombinase Susan Dymecki Mitotic recombination Recombinase-mediated cassette exchange FLP recombinase Site-specific recombination Tetracycline-controlled transcriptional activation Plasmodium molecular tools Site-specific recombinase technology
is Wikipage redirect of	FLP-FRT Recombination Flippase recognition target FLP recombinase
is foaf:primaryTopic of	wikipedia-en:FLP-FRT_recombination

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