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About: Fura-2     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : umbel-rc:ChemicalSubstanceType, within Data Space : dbpedia.demo.openlinksw.com associated with source document(s)

Type: Thingdul:ChemicalObjectchemical substanceChemical compoundyago:WikicatBenzofuransyago:WikicatBiochemistryMethodsyago:WikicatSaltsAndEstersOfCarboxylicAcidsyago:Ability105616246yago:AbsorptionIndicator114598383yago:Abstraction100002137yago:Benzofuran114898101yago:Chemical114806838yago:Cognition100023271yago:ColoringMaterial114984973yago:Compound105870180yago:Compound114818238yago:Concept105835747yago:Content105809192yago:Dye114985383yago:Element114840755yago:Fluorescein114987695yago:Idea105833840yago:Indicator114917208yago:Know-how105616786yago:Material114580897yago:Matter100020827yago:Method105660268yago:Part113809207yago:PhysicalEntity100001930yago:PsychologicalFeature100023100yago:Quintessence114847103yago:Relation100031921chemical compoundyago:Salt115010703yago:Substance100019613yago:Whole105869584yago:WikicatFluorescentDyesyago:WikicatPhenolEthersyago:WikicatPotassiumCompoundsumbel-rc:ChemicalSubstanceType New Facet based on Instances of this Class

Fura-2, an aminopolycarboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium. It was the first widely used dye for calcium imaging, and remains very popular. Fura-2 is excited at 340 nm and 380 nm of light, and the ratio of the emissions at those wavelengths is directly related to the amount of intracellular calcium. Regardless of the presence of calcium, Fura-2 emits at 510 nm of light. The use of the ratio automatically cancels out confounding variables, such as variable dye concentration and cell thickness, making Fura-2 one of the most appreciated tools to quantify calcium levels. The high photon yield of fura-2 allowed the first real time (video rate) measurements of calcium inside living cells in 1986. More recently, genetically-encoded calcium indi