Hexadimethrine bromide (commercial brand name Polybrene) is a cationic polymer with several uses. Currently, it is primarily used to increase the efficiency of transduction of certain cells with retrovirus in cell culture. Hexadimethrine bromide acts by neutralizing the charge repulsion between virions and sialic acid on the cell surface. Use of Polybrene can improve transduction efficiency 100-1000 fold although it can be toxic to some cell types. Polybrene in combination with DMSO shock is used to transfect some cell types such as NIH-3T3 and CHO. It has other uses, including a role in protein sequencing.
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| - Heksadimetrin bromida (in)
- Hexadimethrine bromide (en)
- Полибрен (ru)
- 聚凝胺 (zh)
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| - Полибрен (гексадиметрина бромид) — катионный полимер, используемый для повышения эффективности заражения ретровирусами эукариотических клеток в клеточной культуре. Полибрен нейтрализует заряды отталкивания между вирионами и сиаловой кислотой на поверхности клетки.. Так же используется при секвенировании белков. (ru)
- 聚凝胺(Polybrene)，或稱為hexadimethrine bromide。它是一種陽離子聚合物，常用於哺乳動物細胞的DNA感染實驗以增強脂質體的感染效率。 目前廣泛用於反轉錄病毒介導的基因感染，慢病毒介導的基因感染，作用機理可能是通過中和細胞表面唾液酸與病毒顆粒之間的靜電排斥從而促進吸附作用。其也是一種有名的抗肝素劑（），常用來生產非特異性凝集的紅細胞。另外也多用於蛋白測序，因為小劑量的聚凝胺在自動測序分析可明顯改善多胜肽的降解現象。 (zh)
- Hexadimethrine bromide (commercial brand name Polybrene) is a cationic polymer with several uses. Currently, it is primarily used to increase the efficiency of transduction of certain cells with retrovirus in cell culture. Hexadimethrine bromide acts by neutralizing the charge repulsion between virions and sialic acid on the cell surface. Use of Polybrene can improve transduction efficiency 100-1000 fold although it can be toxic to some cell types. Polybrene in combination with DMSO shock is used to transfect some cell types such as NIH-3T3 and CHO. It has other uses, including a role in protein sequencing. (en)
- Heksadimetrin bromida (merek dagang Polybrene) adalah polimer kationik yang digunakan untuk meningkatkan efisiensi transduksi sel-sel tertentu dengan retrovirus dalam kultur sel. Heksadimetrin bromida bekerja dengan menetralkan tolak muatan antara virion dan asam silikat pada permukaan sel. Penggunaan Polybrene dapat meningkatkan efisiensi transduksi 100-1000 kali lipat meskipun dapat menjadi toksik bagi beberapa jenis sel. Polybrene dalam kombinasi dengan syok DMSO digunakan untuk mentransfeksi beberapa tipe sel seperti NIH-3T3 dan CHO. Senyawa ini memiliki kegunaan lain, termasuk peran dalam sekuensing protein. (in)
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| - Hexadimethrine bromide (commercial brand name Polybrene) is a cationic polymer with several uses. Currently, it is primarily used to increase the efficiency of transduction of certain cells with retrovirus in cell culture. Hexadimethrine bromide acts by neutralizing the charge repulsion between virions and sialic acid on the cell surface. Use of Polybrene can improve transduction efficiency 100-1000 fold although it can be toxic to some cell types. Polybrene in combination with DMSO shock is used to transfect some cell types such as NIH-3T3 and CHO. It has other uses, including a role in protein sequencing. Hexadimethrine bromide also reverses heparin anticoagulation during open-heart surgery, and it was the original reversal agents used in the 1950s and 1960s. It was replaced by protamine sulfate in 1969, after it was shown that hexadimethrine bromide could potentially cause kidney failure in dogs when used in doses in excess of its therapeutic range. It is still used as an alternative to protamine sulfate for patients who are sensitive to protamine, and at least one surgical center has gone back to using it as their standard reversal agent, since protamine sulfate causes at least a mild hypotensive reaction in most or all patients Hexadimethrine bromide is also used in enzyme kinetic assays in order to reduce spontaneous activation of zymogens that are prone to auto activation. (en)
- Heksadimetrin bromida (merek dagang Polybrene) adalah polimer kationik yang digunakan untuk meningkatkan efisiensi transduksi sel-sel tertentu dengan retrovirus dalam kultur sel. Heksadimetrin bromida bekerja dengan menetralkan tolak muatan antara virion dan asam silikat pada permukaan sel. Penggunaan Polybrene dapat meningkatkan efisiensi transduksi 100-1000 kali lipat meskipun dapat menjadi toksik bagi beberapa jenis sel. Polybrene dalam kombinasi dengan syok DMSO digunakan untuk mentransfeksi beberapa tipe sel seperti NIH-3T3 dan CHO. Senyawa ini memiliki kegunaan lain, termasuk peran dalam sekuensing protein. Sejarahnya, heksadimetrin bromida terkadang digunakan untuk membalikkan antikoagulasi heparin selama operasi jantung terbuka. Saat ini, penggunaannya telah digantikan oleh . Polybrene juga digunakan dalam uji kinetik enzim untuk mengurangi aktivasi zimogen spontan yang rentan terhadap aktivasi otomatis. (in)
- Полибрен (гексадиметрина бромид) — катионный полимер, используемый для повышения эффективности заражения ретровирусами эукариотических клеток в клеточной культуре. Полибрен нейтрализует заряды отталкивания между вирионами и сиаловой кислотой на поверхности клетки.. Так же используется при секвенировании белков. (ru)
- 聚凝胺(Polybrene)，或稱為hexadimethrine bromide。它是一種陽離子聚合物，常用於哺乳動物細胞的DNA感染實驗以增強脂質體的感染效率。 目前廣泛用於反轉錄病毒介導的基因感染，慢病毒介導的基因感染，作用機理可能是通過中和細胞表面唾液酸與病毒顆粒之間的靜電排斥從而促進吸附作用。其也是一種有名的抗肝素劑（），常用來生產非特異性凝集的紅細胞。另外也多用於蛋白測序，因為小劑量的聚凝胺在自動測序分析可明顯改善多胜肽的降解現象。 (zh)
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| - 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide (en)
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