High-performance thin-layer chromatography (HPTLC) is an enhanced form of thin-layer chromatography (TLC). A number of enhancements can be made to the basic method of thin-layer chromatography to automate the different steps, to increase the resolution achieved, and to allow more accurate quantitative measurements. Automation is useful to overcome the uncertainty in droplet size and position when the sample is applied to the TLC plate by hand. One approach to automation has been the use of piezoelectric devices and inkjet printers for applying the sample.
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| - Hochleistungsdünnschichtchromatographie (de)
- High-performance thin-layer chromatography (en)
- Cromatografia su strato sottile ad alta prestazione (it)
- Высокоэффективная тонкослойная хроматография (ru)
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| - Die Hochleistungsdünnschichtchromatographie (HPTLC von engl. high-performance thin-layer chromatography) ist ein physikalisch-chemisches Trennverfahren und eine Weiterentwicklung der klassischen Dünnschichtchromatographie (DC), bei dem Hochleistungstrennschichten unter Einsatz von Geräten verwendet werden. Unter den planar-chromatographischen Methoden, zu denen noch die Papierchromatographie und die DC zählen, ist die HPTLC derzeit die leistungsstärkste. (de)
- Высокоэффективная тонкослойная хроматография (ВЭТСХ, англ. HPTLC) – это физико-химический метод анализа, усовершенствованная форма тонкослойной хроматографии (ТСХ), где разделение веществ осуществляется в высокоэффективных тонких слоях с помощью автоматизированного оборудования, обеспечивая улучшенную разделительную эффективность. По эффективности разделения и воспроизводимости метод высокоэффективной тонкослойной хроматографии превосходит прочие методы планарной хроматографии – бумажную и тонкослойную хроматографии. (ru)
- High-performance thin-layer chromatography (HPTLC) is an enhanced form of thin-layer chromatography (TLC). A number of enhancements can be made to the basic method of thin-layer chromatography to automate the different steps, to increase the resolution achieved, and to allow more accurate quantitative measurements. Automation is useful to overcome the uncertainty in droplet size and position when the sample is applied to the TLC plate by hand. One approach to automation has been the use of piezoelectric devices and inkjet printers for applying the sample. (en)
- La cromatografia su strato sottile ad alta prestazione o HPTLC, acronimo dell'inglese High Performance Thin Layer Chromatography, è una tecnica cromatografica che costituisce un perfezionamento ad alta prestazione della cromatografia su strato sottile classica. (it)
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| - Die Hochleistungsdünnschichtchromatographie (HPTLC von engl. high-performance thin-layer chromatography) ist ein physikalisch-chemisches Trennverfahren und eine Weiterentwicklung der klassischen Dünnschichtchromatographie (DC), bei dem Hochleistungstrennschichten unter Einsatz von Geräten verwendet werden. Unter den planar-chromatographischen Methoden, zu denen noch die Papierchromatographie und die DC zählen, ist die HPTLC derzeit die leistungsstärkste. (de)
- High-performance thin-layer chromatography (HPTLC) is an enhanced form of thin-layer chromatography (TLC). A number of enhancements can be made to the basic method of thin-layer chromatography to automate the different steps, to increase the resolution achieved, and to allow more accurate quantitative measurements. Automation is useful to overcome the uncertainty in droplet size and position when the sample is applied to the TLC plate by hand. One approach to automation has been the use of piezoelectric devices and inkjet printers for applying the sample. The spot capacity (analogous to peak capacity in HPLC) can be increased by developing the plate with two different solvents, using two-dimensional chromatography. The procedure begins with development of sample loaded plate with first solvent. After removing it, the plate is rotated 90° and developed with a second solvent. (en)
- La cromatografia su strato sottile ad alta prestazione o HPTLC, acronimo dell'inglese High Performance Thin Layer Chromatography, è una tecnica cromatografica che costituisce un perfezionamento ad alta prestazione della cromatografia su strato sottile classica. La fase stazionaria è generalmente costituita da particelle di silice con granulometria di 5 - 6 µm, contro i 10 - 12 µm di quelle utilizzate nella TLC classica. Non solo la granulometria delle particelle è ridotta ma anche la geometria delle stesse è ottimizzata. Questo comporta un aumento della selettività e della efficienza nella separazione cromatografica. (it)
- Высокоэффективная тонкослойная хроматография (ВЭТСХ, англ. HPTLC) – это физико-химический метод анализа, усовершенствованная форма тонкослойной хроматографии (ТСХ), где разделение веществ осуществляется в высокоэффективных тонких слоях с помощью автоматизированного оборудования, обеспечивая улучшенную разделительную эффективность. По эффективности разделения и воспроизводимости метод высокоэффективной тонкослойной хроматографии превосходит прочие методы планарной хроматографии – бумажную и тонкослойную хроматографии. (ru)
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