Holtfreter's solution (Holtfreter's medium) is a balanced salt solution that was developed by the developmental biologist Johannes Holtfreter for studying amphibian embryos and to reduce bacterial infections. As a specialised aqueous solution, it finds use in aquaria to prevent infections for early stage amphibians, where it is typically mixed with soft tap water. Amphibians such as axolotls prefer a hard water solution.
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