The Holy Trinity Serbian Orthodox Church is a Serbian Orthodox Church located in Regina, Saskatchewan. It is part of the Serbian Orthodox Eparchy of Canada. It is located at 928 Eleventh Avenue and Winnipeg Street in Regina. It has the distinction of being the first Serbian church to be built in the Dominion of Canada, being built in 1912. The second oldest Serbian church in Canada is the on Barton Street in Hamilton, inaugurated in 1917.