Johanna of Hanau-Lichtenberg, Countess of Eberstein (1507 – 27 January 1572) was a German noblewoman, the eldest daughter of Philipp III, Count of Hanau-Lichtenberg (18 October 1482 – 15 May 1538) and Margravine Sibylle of Baden (26 April 1485 – 10 July 1518).