John Twynyho (c. 1440 – 30 September 1485) (alias Twynyhoe, Twynihoe, etc.) of Cirencester, Bristol and Lechlade, all in Gloucestershire, was a lawyer and wealthy wool merchant who served as Recorder of Bristol, as a Member of Parliament for Bristol in Gloucestershire in 1472-5 and in 1484 and for the prestigious county seat Gloucestershire in 1476. In 1478 he was Attorney General to Lord Edward (the future King Edward V (1483–1483)), eldest son and heir of King Edward IV.