Mud Creek is a tributary of Chillisquaque Creek in Columbia County and Montour County in Pennsylvania, in the United States. It is approximately 8.3 miles (13.4 km) long and flows through Madison Township, Columbia County and Derry Township, Montour County. The main tributaries of the creek are unnamed tributaries. The creek's watershed has an area of 17.70 square miles and is in West Hemlock Township, Montour County as well as the two townships it flows through. The watershed is mostly agricultural and forested and the main developed areas are Washingtonville and Jerseytown.