State Route 335 (SR 335) is a north–south state highway in the southern portion of the U.S. state of Ohio. Its southern terminus is at US 52 in Sciotoville, a neighborhood within the city of Portsmouth, and its northern terminus is at SR 220 in Waverly where it has a wrong-way concurrency with US 23 and SR 104 for 0.35 miles (0.56 km).