Photopolymerization-based signal amplification (PBA) is a method of amplifying detection signals from molecular recognition events in an immunoassay by utilizing a radical polymerization initiated through illumination by light. To contrast between a negative and a positive result, PBA is linked to a colorimetric method, thereby resulting in a change in color when a targeted analyte is detected, i.e., a positive signal. PBA is also used to quantify the concentration of the analyte by measuring intensity of the color.
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