About: Propidium monoazide

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Propidium monoazide (PMA) is a photoreactive DNA-binding dye that preferentially binds to dsDNA. It is used to detect viable microorganisms by qPCR. Visible light (high power halogen lamps or specific LED devices) induces a photoreaction of the chemical that will lead to a covalent bond with PMA and the dsDNA. The mechanism of DNA modification by PMA can be seen in this protocol. This process renders the DNA insoluble and results in its loss during subsequent genomic DNA extraction. Theoretically, dead microorganisms lose their capability to maintain their membranes intact, which leaves the "naked" DNA in the cytosol ready to react with PMA. DNA of living organisms are not exposed to the PMA, as they have an intact cell membrane. After treatment with the chemical, only the DNA from living

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rdf:type	Thing dul:ChemicalObject chemical substance Chemical compound yago:WikicatAzides yago:Abstraction100002137 yago:Azide114770248 yago:Chemical114806838 yago:ColoringMaterial114984973 yago:Compound114818238 yago:Dye114985383 yago:Material114580897 yago:Matter100020827 yago:Part113809207 yago:PhysicalEntity100001930 yago:Relation100031921 chemical compound yago:Substance100019613 yago:WikicatDyes
rdfs:label	Propidium monoazide (en)
rdfs:comment	Propidium monoazide (PMA) is a photoreactive DNA-binding dye that preferentially binds to dsDNA. It is used to detect viable microorganisms by qPCR. Visible light (high power halogen lamps or specific LED devices) induces a photoreaction of the chemical that will lead to a covalent bond with PMA and the dsDNA. The mechanism of DNA modification by PMA can be seen in this protocol. This process renders the DNA insoluble and results in its loss during subsequent genomic DNA extraction. Theoretically, dead microorganisms lose their capability to maintain their membranes intact, which leaves the "naked" DNA in the cytosol ready to react with PMA. DNA of living organisms are not exposed to the PMA, as they have an intact cell membrane. After treatment with the chemical, only the DNA from living (en)
foaf:depiction
dcterms:subject	Organoazides Molecular biology DNA-binding substances Dyes
Wikipage page ID	40882801 (xsd:integer)
Wikipage revision ID	1117032474 (xsd:integer)
Link from a Wikipage to another Wikipage	Organoazides Molecular biology DNA-binding substances Flow cytometry Dyes LED DsDNA Microorganisms Viability PCR Photoreactive QPCR Fluorescence microscopy
sameAs	Propidium monoazide Propidium monoazide Propidium monoazide Propidium monoazide
dbp:wikiPageUsesTemplate	dbt:Chembox dbt:Chembox_Hazards dbt:Chembox_Identifiers dbt:Chembox_Properties dbt:Reflist
thumbnail	wiki-commons:Special:FilePath/Propidium_monoazide.svg?width=300
ImageFile	Propidium monoazide-02.png (en) Propidium monoazide.svg (en)
imagesize	200 (xsd:integer)
IUPACName	3 (xsd:integer)
has abstract	Propidium monoazide (PMA) is a photoreactive DNA-binding dye that preferentially binds to dsDNA. It is used to detect viable microorganisms by qPCR. Visible light (high power halogen lamps or specific LED devices) induces a photoreaction of the chemical that will lead to a covalent bond with PMA and the dsDNA. The mechanism of DNA modification by PMA can be seen in this protocol. This process renders the DNA insoluble and results in its loss during subsequent genomic DNA extraction. Theoretically, dead microorganisms lose their capability to maintain their membranes intact, which leaves the "naked" DNA in the cytosol ready to react with PMA. DNA of living organisms are not exposed to the PMA, as they have an intact cell membrane. After treatment with the chemical, only the DNA from living bacteria is usable in qPCR, allowing to obtain only the amplified DNA of living organisms. This is helpful in determining which pathogens are active in specific samples. The main use of PMA is in Viability PCR but the same principle can be applied in flow cytometry or fluorescence microscopy. However, the ability of PMA in differentiating viable and non-viable cells varies for different bacteria. An example is that the permeability of PMA to gram-positive and gram-negative cell membranes is different. Therefore, the application of PMA to mixed communities is still limited. PMA was developed at Biotium, Inc. as an improvement on ethidium monoazide (EMA). PMA provides better discrimination between live and dead bacteria because it is excluded from live cells more efficiently than EMA. (en)
gold:hypernym	Dye
prov:wasDerivedFrom	wikipedia-en:Propidium_monoazide?oldid=1117032474&ns=0
page length (characters) of wiki page	5174 (xsd:nonNegativeInteger)
IUPAC name	3-Amino-8-azido-5-{3-[diethyl(methyl)ammonio]propyl}-6-phenylphenanthridinium (en)
foaf:isPrimaryTopicOf	wikipedia-en:Propidium_monoazide
is Link from a Wikipage to another Wikipage of	C27H32N6 PMA Ethidium bromide Viability PCR PMA dye
is Wikipage redirect of	PMA dye
is foaf:primaryTopic of	wikipedia-en:Propidium_monoazide

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