Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis. The original was isolated from Discosoma, and named DsRed. Others are now available that fluoresce orange, red, and far-red. RFP is approximately 25.9 kDa. The is 558 nm, and the is 583 nm. DsRed has been shown to be more suitable for optical imaging approaches than EGFP.
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| - Red fluorescent protein (en)
- Proteína fluorescente vermelha (pt)
- Красный флуоресцентный белок (ru)
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| - Красный флуоресцентный белок (КФБ) (англ. Red fluorescent protein, RFP) — белок-флуорофор, выделенный из кораллов рода Discosoma отряда морских анемон, который при возбуждении флуоресцирует в красно-оранжевом диапазоне. Кроме этого, с помощью направленного мутагенеза из натурального белка было получено несколько вариантов с различными спектральными характеристиками, включая варианты, флуоресцирующие в оранжевом, красном и инфракрасном диапазоне. (ru)
- Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis. The original was isolated from Discosoma, and named DsRed. Others are now available that fluoresce orange, red, and far-red. RFP is approximately 25.9 kDa. The is 558 nm, and the is 583 nm. DsRed has been shown to be more suitable for optical imaging approaches than EGFP. (en)
- Proteína fluorescente vermelha (do inglês Red Fluorescent Protein, RFP) é um fluoróforo que apresenta fluorescências laranja-vermelha quando excitado. Várias variantes foram desenvolvidas usando mutagênese dirigida. O original foi isolado de Discosoma e denominado DsRed. Outros estão agora disponíveis com fluorescência em laranja, vermelho e vermelho extremo. RFP é de aproximadamente 25,9 kDa. O máximo de excitação é 558 nm, e a emissão máxima é 583 nm. DsRed demonstrou ser mais adequado para abordagens de imagens ópticas do que EGFP . (pt)
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| - Red fluorescent protein drFP583 (en)
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| - Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis. The original was isolated from Discosoma, and named DsRed. Others are now available that fluoresce orange, red, and far-red. RFP is approximately 25.9 kDa. The is 558 nm, and the is 583 nm. The first fluorescent protein to be discovered, green fluorescent protein (GFP), has been adapted to identify and develop fluorescent markers in other colors. Variants such as yellow fluorescent protein (YFP) and cyan fluorescent protein (CFP) were discovered in Anthozoa. Issues with fluorescent proteins include the length of time between protein synthesis and expression of fluorescence. DsRed has an maturation time of around 24 hours, which can make it unusable for many experiments that take place in a shorter time frame. Additionally, DsRed exists in a tetrameric form, which can affect the function of proteins to which it is attached. Genetic engineering has improved the utility of RFP by increasing the speed of fluorescent development and creating monomeric variants. Improved variants of RFP include mFruits (mCherry, mOrange, mRaspberry), mKO, TagRFP, mKate, mRuby, FusionRed, mScarlet and DsRed-Express. DsRed has been shown to be more suitable for optical imaging approaches than EGFP. (en)
- Proteína fluorescente vermelha (do inglês Red Fluorescent Protein, RFP) é um fluoróforo que apresenta fluorescências laranja-vermelha quando excitado. Várias variantes foram desenvolvidas usando mutagênese dirigida. O original foi isolado de Discosoma e denominado DsRed. Outros estão agora disponíveis com fluorescência em laranja, vermelho e vermelho extremo. RFP é de aproximadamente 25,9 kDa. O máximo de excitação é 558 nm, e a emissão máxima é 583 nm. A primeira proteína fluorescente a ser descoberta, a proteína fluorescente verde (GFP), foi adaptada para identificar e desenvolver marcadores fluorescentes em outras cores. Variantes como a proteína fluorescente amarela (YFP) e a proteína fluorescente ciano (CFP) foram descobertas em Anthozoa. Problemas com incluem o período de tempo entre a síntese de proteínas e a expressão de fluorescência. O DsRed tem um tempo de maturação em torno de 24 horas, que pode torná-lo inutilizável para muitos experimentos que ocorrem em um período de tempo mais curto. Além disso, o DsRed existe em uma forma tetramérica, o que pode afetar a função das proteínas às quais está ligado. A engenharia genética melhorou a utilidade do RFP, aumentando a velocidade de desenvolvimento fluorescente e criando variantes monoméricas. DsRed demonstrou ser mais adequado para abordagens de imagens ópticas do que EGFP . (pt)
- Красный флуоресцентный белок (КФБ) (англ. Red fluorescent protein, RFP) — белок-флуорофор, выделенный из кораллов рода Discosoma отряда морских анемон, который при возбуждении флуоресцирует в красно-оранжевом диапазоне. Кроме этого, с помощью направленного мутагенеза из натурального белка было получено несколько вариантов с различными спектральными характеристиками, включая варианты, флуоресцирующие в оранжевом, красном и инфракрасном диапазоне. (ru)
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