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About: STED microscopy     Goto   Sponge   NotDistinct   Permalink

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Type: yago:WikicatMicroscopesyago:Artifact100021939yago:Device103183080yago:Instrument103574816yago:Instrumentality103575240yago:Magnifier103709206yago:Microscope103760671yago:Object100002684yago:PhysicalEntity100001930weaponyago:ScientificInstrument104147495yago:Whole100003553 New Facet based on Instances of this Class

Stimulated emission depletion (STED) microscopy is one of the techniques that make up super-resolution microscopy. It creates super-resolution images by the selective deactivation of fluorophores, minimizing the area of illumination at the focal point, and thus enhancing the achievable resolution for a given system. It was developed by Stefan W. Hell and Jan Wichmann in 1994, and was first experimentally demonstrated by Hell and Thomas Klar in 1999. Hell was awarded the Nobel Prize in Chemistry in 2014 for its development. In 1986, V.A. Okhonin (Institute of Biophysics, USSR Academy of Sciences, Siberian Branch, Krasnoyarsk) had patented the STED idea. This patent was unknown to Hell and Wichmann in 1994.