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About: Strep-tag     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : yago:WikicatProteinMethods, within Data Space : dbpedia.demo.openlinksw.com associated with source document(s)

Type: softwareyago:WikicatLaboratoryTechniquesyago:WikicatMolecularBiologyTechniquesyago:Ability105616246yago:Abstraction100002137yago:Cognition100023271yago:Know-how105616786yago:Method105660268yago:PsychologicalFeature100023100yago:Technique105665146yago:WikicatProteinMethods New Facet based on Instances of this Class

The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin, and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions, it is especially suited for generation of functional proteins.