Thiosulfate-citrate-bile salts-sucrose agar, or TCBS agar, is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio species. TCBS agar is highly selective for the isolation of V. cholerae and V. parahaemolyticus as well as other Vibrio species. Apart from TCBS other rapid testing dipsticks like Immunochromatographic dipstick is also used in endemic areas such as Asia, Africa and Latin America. Though, TCBS agar study is required for confirmation. This becomes immensely important in cases of gastroenteritis caused by campylobacter species, whose symptoms mimic that of cholera. Since no yellow bacterial growth is observed in case of Campylobacter species on TCBS agar, chances of incorrect diagnosis can be rectified. TCBS agar contains high concent
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| - TCBS-Agar (de)
- Milieu TCBS (fr)
- Terreno di Monsur (it)
- TCBS (pl)
- Thiosulfate-citrate-bile salts-sucrose agar (en)
- 硫代硫酸鹽-檸檬酸鹽-膽鹽-蔗糖洋菜培養基 (zh)
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| - Le milieu TCBS (ou Gélose TCBS ou milieu Thiosulfate Citrate Bile Saccharose) est un milieu de culture et d'isolement sélectif des bactéries du genre Vibrio. (fr)
- Il Terreno di Monsur (in inglese Thiosulfate-citrate-bile salts-sucrose agar o TCBS) è un terreno di coltura specifico per la crescita dei vibrioni, molto selettivo per l'isolamento di Vibrio cholerae e Vibrio parahaemolyticus nonché di altre specie del genere Vibrio, per via del suo pH alcalino. Contiene alcune sostanze che inibiscono la crescita sia dei batteri Gram-positivi che della maggior parte di quelli Gram-negativi. Sono usati per rilevare il cambiamento di pH il blu di timolo ed il blu di bromotimolo. (it)
- TCBS (z ang. thiosulfate-citrate-bile salts-sucrose agar) – podłoże mikrobiologiczne zawierające tiosiarczan sodu, cytrynian sodu, sole żółciowe i sacharozę. Służy gł. do hodowli i różnicowania bakterii rodzaju Vibrio. (pl)
- 硫代硫酸鹽-檸檬酸鹽-膽鹽-蔗糖洋菜培養基（Thiosulfate-citrate-bile salts-sucrose agar，簡稱TCBS培養基），也稱為弧菌選擇培養基，是一種用來檢驗弧菌種類的培養基。無法發酵蔗糖的細菌，例如腸炎弧菌，在此培養基上的菌落顏色會呈現綠色；而能夠發酵蔗糖的細菌菌落則呈現黃色。TCBS培養基的主要成分是硫代硫酸鈉、、檸檬酸鈉和蔗糖。培养基还包含百里酚蓝和溴百里酚藍作为pH指示剂以显示培养细菌时的酸碱值变化。 (zh)
- Der TCBS-Agar ist ein Nährmedium zur Isolierung und selektiven Kultivierung von Bakterien der Gattung Vibrio, wie z. B. den Erreger der Cholera – Vibrio cholerae – und anderer Vibrionen, die Brechdurchfall-Erkrankungen (Gastroenteritis) erzeugen, wie Vibrio parahaemolyticus oder die so genannten NAG-Vibrionen (nicht-agglutinierende Vibrionen), bei denen es sich um non-O1-Stämme von Vibrio cholerae handelt. (de)
- Thiosulfate-citrate-bile salts-sucrose agar, or TCBS agar, is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio species. TCBS agar is highly selective for the isolation of V. cholerae and V. parahaemolyticus as well as other Vibrio species. Apart from TCBS other rapid testing dipsticks like Immunochromatographic dipstick is also used in endemic areas such as Asia, Africa and Latin America. Though, TCBS agar study is required for confirmation. This becomes immensely important in cases of gastroenteritis caused by campylobacter species, whose symptoms mimic that of cholera. Since no yellow bacterial growth is observed in case of Campylobacter species on TCBS agar, chances of incorrect diagnosis can be rectified. TCBS agar contains high concent (en)
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| - Der TCBS-Agar ist ein Nährmedium zur Isolierung und selektiven Kultivierung von Bakterien der Gattung Vibrio, wie z. B. den Erreger der Cholera – Vibrio cholerae – und anderer Vibrionen, die Brechdurchfall-Erkrankungen (Gastroenteritis) erzeugen, wie Vibrio parahaemolyticus oder die so genannten NAG-Vibrionen (nicht-agglutinierende Vibrionen), bei denen es sich um non-O1-Stämme von Vibrio cholerae handelt. TCBS-Agar steht für Thiosulfate Citrate Bile (Salts) Sucrose Agar und verweist auf die wichtigsten Komponenten des Nährmediums: Natriumthiosulfat, Natriumcitrat, Gallensalze (im Englischen bile für Galle) und Saccharose (im Englischen sucrose). Die ursprünglich (1962) veröffentlichte Rezeptur des Japaners Y. Nakanishi wurde ein Jahr später durch T. Kobayashi modifiziert. (de)
- Le milieu TCBS (ou Gélose TCBS ou milieu Thiosulfate Citrate Bile Saccharose) est un milieu de culture et d'isolement sélectif des bactéries du genre Vibrio. (fr)
- Thiosulfate-citrate-bile salts-sucrose agar, or TCBS agar, is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio species. TCBS agar is highly selective for the isolation of V. cholerae and V. parahaemolyticus as well as other Vibrio species. Apart from TCBS other rapid testing dipsticks like Immunochromatographic dipstick is also used in endemic areas such as Asia, Africa and Latin America. Though, TCBS agar study is required for confirmation. This becomes immensely important in cases of gastroenteritis caused by campylobacter species, whose symptoms mimic that of cholera. Since no yellow bacterial growth is observed in case of Campylobacter species on TCBS agar, chances of incorrect diagnosis can be rectified. TCBS agar contains high concentrations of sodium thiosulfate and sodium citrate to inhibit the growth of Enterobacteriaceae. Inhibition of Gram-positive bacteria is achieved by the incorporation of ox gall, which is a naturally occurring substance containing a mixture of bile salts and sodium cholate, a pure bile salt. Sodium thiosulfate also serves as a sulfur source and its presence, in combination with ferric citrate, allows for the easy detection of hydrogen sulfide production. Saccharose (sucrose) is included as a fermentable carbohydrate for metabolism by Vibrio species. The alkaline pH of the medium enhances the recovery of V. cholerae and inhibits the growth of others. Thymol blue and bromothymol blue are included as indicators of pH changes. (en)
- Il Terreno di Monsur (in inglese Thiosulfate-citrate-bile salts-sucrose agar o TCBS) è un terreno di coltura specifico per la crescita dei vibrioni, molto selettivo per l'isolamento di Vibrio cholerae e Vibrio parahaemolyticus nonché di altre specie del genere Vibrio, per via del suo pH alcalino. Contiene alcune sostanze che inibiscono la crescita sia dei batteri Gram-positivi che della maggior parte di quelli Gram-negativi. Sono usati per rilevare il cambiamento di pH il blu di timolo ed il blu di bromotimolo. (it)
- TCBS (z ang. thiosulfate-citrate-bile salts-sucrose agar) – podłoże mikrobiologiczne zawierające tiosiarczan sodu, cytrynian sodu, sole żółciowe i sacharozę. Służy gł. do hodowli i różnicowania bakterii rodzaju Vibrio. (pl)
- 硫代硫酸鹽-檸檬酸鹽-膽鹽-蔗糖洋菜培養基（Thiosulfate-citrate-bile salts-sucrose agar，簡稱TCBS培養基），也稱為弧菌選擇培養基，是一種用來檢驗弧菌種類的培養基。無法發酵蔗糖的細菌，例如腸炎弧菌，在此培養基上的菌落顏色會呈現綠色；而能夠發酵蔗糖的細菌菌落則呈現黃色。TCBS培養基的主要成分是硫代硫酸鈉、、檸檬酸鈉和蔗糖。培养基还包含百里酚蓝和溴百里酚藍作为pH指示剂以显示培养细菌时的酸碱值变化。 (zh)
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