The Toledo Subdivision is a railroad line owned and operated by CSX Transportation in the U.S. state of Ohio. The line runs from Hamilton (north of Cincinnati) north to Perrysburg (near Toledo) along a former Baltimore and Ohio Railroad line. The south end of the Toledo Subdivision is at the north end of the Cincinnati Terminal Subdivision, near the east end of the Indianapolis Subdivision. It's north end is at the south end of the . In between, it junctions with the Middletown Subdivision at New Miami, the at Sidney, and the Garrett Subdivision at Deshler.