About: Virtual colony count

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Virtual colony count (VCC) is a kinetic, 96-well microbiological assay originally developed to measure the activity of defensins. It has since been applied to other antimicrobial peptides including LL-37. It utilizes a method of enumerating bacteria called quantitative growth kinetics, which compares the time taken for a bacterial batch culture to reach a threshold optical density with that of a series of calibration curves. The name VCC has also been used to describe the application of quantitative growth kinetics to enumerate bacteria in cell culture infection models.Antimicrobial susceptibility testing (AST) can be done on 96-well plates by diluting the antimicrobial agent at varying concentrations in broth inoculated with bacteria and measuring the minimum inhibitory concentration that

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rdf:type	yago:Ability105616246 yago:Abstraction100002137 yago:Cognition100023271 yago:Know-how105616786 yago:Method105660268 yago:PsychologicalFeature100023100 yago:WikicatResearchMethods
rdfs:label	Virtual colony count (en)
rdfs:comment	Virtual colony count (VCC) is a kinetic, 96-well microbiological assay originally developed to measure the activity of defensins. It has since been applied to other antimicrobial peptides including LL-37. It utilizes a method of enumerating bacteria called quantitative growth kinetics, which compares the time taken for a bacterial batch culture to reach a threshold optical density with that of a series of calibration curves. The name VCC has also been used to describe the application of quantitative growth kinetics to enumerate bacteria in cell culture infection models.Antimicrobial susceptibility testing (AST) can be done on 96-well plates by diluting the antimicrobial agent at varying concentrations in broth inoculated with bacteria and measuring the minimum inhibitory concentration that (en)
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dcterms:subject	Research methods
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Wikipage revision ID	1060935985 (xsd:integer)
Link from a Wikipage to another Wikipage	Enantiomer Biofilms Antimicrobial Antimicrobial peptides Beta-defensin 2 Beta bulge dbr:DEFA5 Electrostatic Research methods Glycine Anthrax Staphylococcus aureus Pathogen Microplate Mutagenesis Bacteria Bactericidal Buffer solution Cell (biology) Cell membranes Gram-negative Gram-positive Aerosols Alanine Escherichia coli American Type Culture Collection Bioaerosols Defensins Enterobacter aerogenes Microbiological culture Dimer (chemistry) Plate reader Quality control Real-time polymerase chain reaction Bacillus anthracis Bacillus cereus Bacterial growth Acinetobacter baumannii Alanine scanning Binary fission Biosafety Biosafety cabinet Turbidity Assay D-amino acids HD6 HNP1 HNP2 HNP3 HNP4 Minimum inhibitory concentration Exponential growth Cell walls Microbiological Bacteriostatic LL-37 Propeptide Hydrophobicity QPCR Alpha defensins Mutated Pipetting Beta defensins Theta defensins
Link from a Wikipage to an external page	https://www.cdc.gov/biosafety/publications/bmbl5/index.htm
sameAs	Virtual colony count Virtual colony count Virtual colony count Virtual colony count
dbp:wikiPageUsesTemplate	dbt:Reflist dbt:Academic_peer_reviewed
thumbnail	wiki-commons:Special:FilePath/Virtual_Colony_Count_(VCC)_sextuplicate_calibration_growth_curves.png?width=300
has abstract	Virtual colony count (VCC) is a kinetic, 96-well microbiological assay originally developed to measure the activity of defensins. It has since been applied to other antimicrobial peptides including LL-37. It utilizes a method of enumerating bacteria called quantitative growth kinetics, which compares the time taken for a bacterial batch culture to reach a threshold optical density with that of a series of calibration curves. The name VCC has also been used to describe the application of quantitative growth kinetics to enumerate bacteria in cell culture infection models.Antimicrobial susceptibility testing (AST) can be done on 96-well plates by diluting the antimicrobial agent at varying concentrations in broth inoculated with bacteria and measuring the minimum inhibitory concentration that results in no growth. However, these methods cannot be used to study some membrane-active antimicrobial peptides, which are inhibited by the broth itself. The virtual colony count procedure takes advantage of this fact by first exposing bacterial cells to the active antimicrobial agent in a low-salt buffer for two hours, then simultaneously inhibiting antimicrobial activity and inducing exponential growth by adding broth. The growth kinetics of surviving cells can then be monitored using a temperature-controlled plate reader. The time taken for each growth curve to reach a threshold change in optical density is then converted into virtual survival values, which serve as a measure of antimicrobial activity. (en)
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page length (characters) of wiki page	28891 (xsd:nonNegativeInteger)
foaf:isPrimaryTopicOf	wikipedia-en:Virtual_colony_count
is Link from a Wikipage to another Wikipage of	Antimicrobial peptides Assay VCC Virtual Colony Count Qgk Virtual colony counting
is Wikipage redirect of	Virtual Colony Count Qgk Virtual colony counting

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