Vitamin D3 24-hydroxylase (EC 1.14.15.16, CYP24A1) is an enzyme with systematic name calcitriol,NADPH:oxygen oxidoreductase (24-hydroxylating). This enzyme catalyses the following chemical reaction (1) calcitriol + NADPH + H+ + O2 calcitetrol + NADP+ + H2O(2) calcidiol + NADPH + H+ + O2 secalciferol + NADP+ + H2O Vitamin D3 24-hydroxylase is a heme-thiolate enzyme (P-450).
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