The 1960 United States Senate election in Wyoming was held on November 8, 1960. Incumbent Democratic Senator Joseph C. O'Mahoney was first appointed to the U.S. Senate from Wyoming's Class 1 Senate seat in 1934, and was re-elected in 1934, 1940, and 1946 before losing re-election in 1952. He was then elected to the Class 2 Senate seat in 1954. O'Mahoney, in failing health and increasingly limited in his mobility, declined to run for a fifth non-consecutive term in the Senate. The winner of this election, Keith Thomson, died before assuming his elected office.