The 1989–90 Associate Members' Cup, known as the 1989–90 Leyland DAF Cup, was the seventh staging of the Associate Members' Cup, a knock-out competition for English football clubs in the Third Division and the Fourth Division. The winners were Tranmere Rovers and the runners-up were Bristol Rovers. The competition began on 7 November 1989 and ended with the final on 20 May 1990 at Wembley Stadium.