The 2007 Top League Challenge Series was the 2007 edition of the Top League Challenge Series, a second-tier rugby union competition in Japan, in which teams from regionalised leagues competed for promotion to the Top League for the 2007–08 season. The competition was contested from 14 to 27 January 2007. Kyuden Voltex and Mitsubishi Sagamihara DynaBoars won promotion to the 2007–08 Top League, while Honda Heat and Kintetsu Liners progressed to the promotion play-offs.