The 2016 Iowa State Cyclones football team represented Iowa State University in the 2016 NCAA Division I FBS football season. Playing as a member of the Big 12 Conference (Big 12), the team played its home games at Jack Trice Stadium in Ames, Iowa. They were led by first-year head coach Matt Campbell. They finished the season 3–9, 2–7 in Big 12 play to finish in ninth place.