The 2016 National Football Challenge Cup or 2016 Pakistan Football Federation Cup was the 26th season of domestic cup tournament in Pakistani football. 24 teams took part in this competition from 28 January 2016 to 22 February 2016, which took place in Islamabad, Lahore, and Karachi. Khan Research Laboratories were the defending champions. They defended their title after defeating National Bank 1–0 in the final.