The 2021 NCAA Division I Women's Lacrosse Championship was the 39th annual single-elimination tournament to determine the national champion of Division I NCAA women's college lacrosse. The semifinal and championship rounds were played at Johnny Unitas Stadium in Towson, MD from May 28–30, 2021. All other rounds were played at campus sites, usually at the home field of the higher-seeded team, from May 14–22. This marked the return of the tournament after 2020 was cancelled due to the COVID-19 pandemic.