Affitins (commercial name Nanofitins) are artificial proteins with the ability to selectively bind antigens. They are structurally derived from the DNA binding protein , found in Sulfolobus acidocaldarius, a microorganism belonging to the archaeal domain. By randomizing the amino acids on the binding surface of Sac7d and subjecting the resulting protein library to rounds of ribosome display, the affinity can be directed towards various targets, such as peptides, proteins, viruses, and bacteria. Affitins are antibody mimetics and are being developed as an alternative to antibodies as tools in biotechnology. They have also been used as specific inhibitors for various enzymes. Affitins can be utilized in biochemical purification techniques, specifically in affinity chromatography. The ability
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| - Nanofitin (de)
- Affitin (en)
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| - Nanofitine (Affitine) sind künstliche Proteine, die zur Bindung von Antigenen befähigt sind. Affitine sind strukturell vom DNA-bindenden Protein des Archebakteriums Sulfolobus acidocaldarius abgeleitet. Die Fähigkeit auch Proteine zu binden, erhalten diese Moleküle durch Veränderung ihrer oberflächennahen Aminosäuren. Die zur Familie der Antikörpermimetika zählenden Affitine werden als eine mögliche Alternative zu Antikörpern als Werkzeuge in der Biochemie entwickelt. (de)
- Affitins (commercial name Nanofitins) are artificial proteins with the ability to selectively bind antigens. They are structurally derived from the DNA binding protein , found in Sulfolobus acidocaldarius, a microorganism belonging to the archaeal domain. By randomizing the amino acids on the binding surface of Sac7d and subjecting the resulting protein library to rounds of ribosome display, the affinity can be directed towards various targets, such as peptides, proteins, viruses, and bacteria. Affitins are antibody mimetics and are being developed as an alternative to antibodies as tools in biotechnology. They have also been used as specific inhibitors for various enzymes. Affitins can be utilized in biochemical purification techniques, specifically in affinity chromatography. The ability (en)
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| - Nanofitine (Affitine) sind künstliche Proteine, die zur Bindung von Antigenen befähigt sind. Affitine sind strukturell vom DNA-bindenden Protein des Archebakteriums Sulfolobus acidocaldarius abgeleitet. Die Fähigkeit auch Proteine zu binden, erhalten diese Moleküle durch Veränderung ihrer oberflächennahen Aminosäuren. Die zur Familie der Antikörpermimetika zählenden Affitine werden als eine mögliche Alternative zu Antikörpern als Werkzeuge in der Biochemie entwickelt. (de)
- Affitins (commercial name Nanofitins) are artificial proteins with the ability to selectively bind antigens. They are structurally derived from the DNA binding protein , found in Sulfolobus acidocaldarius, a microorganism belonging to the archaeal domain. By randomizing the amino acids on the binding surface of Sac7d and subjecting the resulting protein library to rounds of ribosome display, the affinity can be directed towards various targets, such as peptides, proteins, viruses, and bacteria. Affitins are antibody mimetics and are being developed as an alternative to antibodies as tools in biotechnology. They have also been used as specific inhibitors for various enzymes. Affitins can be utilized in biochemical purification techniques, specifically in affinity chromatography. The ability of Affitins to selectively bind antigens is used to target specific proteins. Scientists have been able to purify human immunoglobulin G (hIgG), bacterial PulD protein, and chicken egg lysozyme using Affitin columns with a high degree of purity. These have the ability to act as specific ligands for the proteins of interest that are needed when the fusion of proteins to polypeptide tags is impossible or carries no advantage, and thus build affinity columns as is the case in the production of biopharmaceuticals. They were immobilized on an agarose matrix and the columns had a high degree of selectivity. In addition to this, antibodies and non-immunoglobin proteins can be purified by using affitins via affinity chromatography. Due to their small size and high solubility, they can be easily produced in large amounts using bacterial expression systems. (en)
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