BglII is a type II restriction endonuclease isolated from certain strains of Bacillus globigii. The principal function of restriction enzymes is the protection of the host genome against foreign DNA, but they may also have some involvement in recombination and transposition.
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| - BglII (en)
- BglII (es)
- BglII (pt)
- BglII (sv)
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| - BglII es una enzima de restricción de tipo II producida por el microorganismo que posee una diana de restricción en el ADN de cadena doble dependiente de una secuencia no metilada, palindrómica y asimétrica, sobre la cual su actividad catalítica hidrolasa genera extremos cohesivos. La diana de restricción puede representarse según este diagrama: (es)
- BglII är ett restriktionsenzym som finns i Bacillus globigii. Dess funktion är att klyva DNA-strängar vid sekvensen AGATCT. Den klyver inte rakt av utan skapar klistriga ändar, det vill säga ena delen skjuter ut. Denna biokemi-relaterade artikel saknar väsentlig information. Du kan hjälpa till genom att lägga till den. (sv)
- BglII é uma enzima de restrição do tipo II, isolada de certos tipos de estirpes de Bacillus globiggi e faz parte do sistema de restrição-modificação. Em biologia molecular, é uma enzima de restrição vulgarmente utilizada. Cria extremidades coesivas no ADN. A sequência de ácidos nucleicos onde a enzima faz o corte é A|GATCT, que é um palíndromo, já que a sua sequência complementar é TCTAG|A. (pt)
- BglII is a type II restriction endonuclease isolated from certain strains of Bacillus globigii. The principal function of restriction enzymes is the protection of the host genome against foreign DNA, but they may also have some involvement in recombination and transposition. (en)
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| - Restriction endonuclease BglII (en)
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| - structure of restriction endonuclease BstYI bound to non-cognate DNA (en)
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| - BglII is a type II restriction endonuclease isolated from certain strains of Bacillus globigii. The principal function of restriction enzymes is the protection of the host genome against foreign DNA, but they may also have some involvement in recombination and transposition. Like most type II restriction enzymes, BglII consists of two identical subunits that form a homodimer around the DNA double helix. Each monomer is 223 amino acids and symmetrically bind both sides of the unique palindromic nucleotide sequence AGATCT, cleaving the scissile phosphodiester bond between the first Adenine and Guanine nucleotides on both strands of the DNA molecule, creating sticky ends with 5' end overhangs. Being a type II restriction enzyme, BglII does not require ATP (adenosine triphosphate) for its enzymatic function, but only requires association with a divalent metal cation, most likely Mg2+. Unlike other restriction enzymes of its class, BglII has been shown to possess some unique structural characteristics, such as a β-sandwich subdomain, and appears to undergo a unique conformational change upon dimerization, but its overall structure and mechanism of catalysis remain consistent with other type II restriction enzymes. Restriction endonucleases play a very important role in modern molecular cloning techniques. Because of their unique recognition/cut sites, restriction enzymes can be used to precisely cut DNA at specific locations in a predictable manner. Once cut, the DNA (usually) possesses so-called "sticky ends", which can then allow the DNA fragment to hybridise into a DNA vector. Ligating enzymes are used to covalently link the desired fragment to the vector for subsequent DNA cloning. (en)
- BglII es una enzima de restricción de tipo II producida por el microorganismo que posee una diana de restricción en el ADN de cadena doble dependiente de una secuencia no metilada, palindrómica y asimétrica, sobre la cual su actividad catalítica hidrolasa genera extremos cohesivos. La diana de restricción puede representarse según este diagrama: (es)
- BglII är ett restriktionsenzym som finns i Bacillus globigii. Dess funktion är att klyva DNA-strängar vid sekvensen AGATCT. Den klyver inte rakt av utan skapar klistriga ändar, det vill säga ena delen skjuter ut. Denna biokemi-relaterade artikel saknar väsentlig information. Du kan hjälpa till genom att lägga till den. (sv)
- BglII é uma enzima de restrição do tipo II, isolada de certos tipos de estirpes de Bacillus globiggi e faz parte do sistema de restrição-modificação. Em biologia molecular, é uma enzima de restrição vulgarmente utilizada. Cria extremidades coesivas no ADN. A sequência de ácidos nucleicos onde a enzima faz o corte é A|GATCT, que é um palíndromo, já que a sua sequência complementar é TCTAG|A. (pt)
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