Charlotte Frances Frederica Spencer, Countess Spencer, VA (née Seymour; 28 September 1835 – 31 October 1903) was a British philanthropist. Born in the London residence of her maternal grandfather, the 1st Marquess of Bristol, she was the youngest daughter of Frederick Charles William Seymour and his second wife Lady Augusta Hervey. In 1858 Charlotte married John Spencer, 5th Earl Spencer; they had no children.