About: DNA barcoding in diet assessment

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DNA barcoding in diet assessment is the use of DNA barcoding to analyse the diet of organisms. and further detect and describe their trophic interactions. This approach is based on the identification of consumed species by characterization of DNA present in dietary samples, e.g. individual food remains, regurgitates, gut and fecal samples, homogenized body of the host organism, target of the diet study (for example with whole body of insects).

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rdfs:label	DNA barcoding in diet assessment (en)
rdfs:comment	DNA barcoding in diet assessment is the use of DNA barcoding to analyse the diet of organisms. and further detect and describe their trophic interactions. This approach is based on the identification of consumed species by characterization of DNA present in dietary samples, e.g. individual food remains, regurgitates, gut and fecal samples, homogenized body of the host organism, target of the diet study (for example with whole body of insects). (en)
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dcterms:subject	DNA barcoding Bioinformatics Ecology
Wikipage page ID	60579575 (xsd:integer)
Wikipage revision ID	1092882245 (xsd:integer)
Link from a Wikipage to another Wikipage	Amplicon Primer (molecular biology) RuBisCO Endangered species List of feeding behaviours Next-generation sequencing Rumen Non-invasive Biomass Algae DNA barcoding DNA barcoding Bias Cytochrome b DNA DNA barcoding DNA fragmentation Internal transcribed spacer Bioinformatics Genetic marker Genus Omnivore Angiosperms Arachnid Lemming Leopard cat Mammal Chloroplast DNA Maturase K Microbial DNA barcoding Brown bear Locus (genetics) Wildlife management DNA sequencing Diet (nutrition) Family (biology) Taxonomic rank Gymnosperm Harbour porpoise Histology Ecology Chamois Herbivore Trophic level Y chromosome Grey seal DNA amplification Insect Metabarcoding Microscopy Carnivore Microsatellite Species Vole Fish DNA barcoding Pollen DNA barcoding Sanger sequencing Taxonomic resolution Gene sequence Ribulose-bisphosphate carboxylase Plant tissue ITS2
sameAs	DNA barcoding in diet assessment DNA barcoding in diet assessment
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thumbnail	wiki-commons:Special:FilePath/Faeces_of_wolf_(Canis_lupus)_collected_in_Sweden.jpg?width=300
has abstract	DNA barcoding in diet assessment is the use of DNA barcoding to analyse the diet of organisms. and further detect and describe their trophic interactions. This approach is based on the identification of consumed species by characterization of DNA present in dietary samples, e.g. individual food remains, regurgitates, gut and fecal samples, homogenized body of the host organism, target of the diet study (for example with whole body of insects). The DNA sequencing approach to be adopted depends on the diet breadth of the target consumer. For organisms feeding on one or only few species, traditional Sanger sequencing techniques can be used. For polyphagous species with diet items more difficult to identify, it is conceivable to determine all consumed species using NGS methodology. The barcode markers utilized for amplification will differ depending on the diet of the target organism. For herbivore diets, the standard DNA barcode loci will differ significantly depending on the plant taxonomic level. Therefore, for identifying plant tissue at the taxonomic family or genus level, the markers rbcL and trn-L-intron are used, which differ from the loci ITS2, matK, trnH-psbA (noncoding intergenic spacer) used to identify diet items to genus and species level. For animal prey, the most broadly used DNA barcode markers to identify diets are the mitochondrial cytocrome C oxydase (COI) and cytochrome b (cytb). When the diet is broad and diverse, DNA metabarcoding is used to identify most of the consumed items. (en)
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page length (characters) of wiki page	29749 (xsd:nonNegativeInteger)
foaf:isPrimaryTopicOf	wikipedia-en:DNA_barcoding_in_diet_assessment
is Link from a Wikipage to another Wikipage of	Algae DNA barcoding DNA barcoding Fungal DNA barcoding Microbial DNA barcoding Marine food web Fish DNA barcoding
is foaf:primaryTopic of	wikipedia-en:DNA_barcoding_in_diet_assessment

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